476 / 2019-02-27 23:05:15

CIPK11-Dependent Phosphorylation Modulates FIT Activity to Promote Arabidopsis Iron Acquisition in Response to Calcium Signaling

calcium signaling, CIPK11, FIT, protein phosphorylation, iron acquisition

全体主题 > Abiotic Stress

Plant growth is influenced by a changing environment. This requires precise and coordinated decisions on plant development, physiology, and stress response. The bHLH transcription factor FIT is a key regulator of Arabidopsis iron (Fe) acquisition and is post-translationally activated upon Fe deficiency. We identified CBL-INTERACTING PROTEIN KINASE CIPK11 as a FIT interactor. Cytosolic Ca2+ concentration and CIPK11 expression are induced by Fe deficiency. cipk11 mutant plants display compromised root Fe mobilization and seed Fe content. We found that Fe uptake is also dependent on the plasma membrane localized CIPK-activating proteins CBL1 and CBL9. CIPK11 phosphorylates FIT at Ser272, and mutation of this target site affects FIT nuclear accumulation, homo-dimerization, interaction with bHLH039, and transcriptional activity, and the plant’s Fe-uptake ability. We propose that Ca2+-triggered CBL1/9-mediated activation of CIPK11 and subsequent phosphorylation of FIT shifts inactive into active FIT, allowing regulatory protein interactions in the nucleus. This biochemical link between Fe deficiency and the cellular Ca2+ decoding machinery represents an environment-sensing mechanism to adjust nutrient uptake.