摘要详情

ID / 提交时间

676 / 2019-04-30 18:14:23

标题

Targeting mechanism of H3K27me3 demethylase REF6 in Arabidopsis

关键字

H3K27me3, Histone demethylase, DNA methylation

主题及专题

Epigenetic Regulation > Epigenetic Regulation

状态

摘要录用

作者

Xian Deng / Institute of Genetics and Developmental Biology, Chinese Academy of Sciences

Qi Qiu / Institute of Genetics and Developmental Biology, Chinese Academy of Sciences

Hailiang Mei / Institute of Genetics and Developmental Biology, Chinese Academy of Sciences

Kaixuan He / Institute of Genetics and Developmental Biology, Chinese Academy of Sciences

Baixing Wu / SUSTC

Jinbiao Ma / Fudan University

Xiaofeng Cao / Institute of Genetics and Developmental Biology, Chinese Academy of Sciences

摘要

RELATIVE OF EARLY FLOWERING 6 (REF6/JMJ12), a Jumonji C (JmjC)-domain-containing H3K27me3 histone demethylase, finds its target loci in Arabidopsis genome by directly recognizing the CTCTGYTY motif via its zinc-finger (ZnF) domains (1, 2). REF6 tends to bind motifs located in active chromatin states and depleted for heterochromatic modifications. However, the underlying mechanism remains unknown. Here, using biochemistry, structural biology, and high-throughput sequencing, we showed that REF6 binding affinity to specific regions is repelled by DNA methylation within CTCTGYTY motif. In addition to CTCTGYTY motif, REF6 also bind to the other two types of motifs. We will discuss how REF6 determine the recognition of different motifs and the biological significance of REF6 in Arabidopsis.