Abstract:Objective To study the secondary metabolites of the mangrove endophytic fungus, Talaromyces sp.10#.Method The structures of these compounds were established by spectroscopic analysis and comparison with related reported data. The absolute configurations were determined by X-ray single crystal diffraction and electronic circular dichroism (ECD) calculation. Results three Cytochalasins, 20-Deoxycytochalasin F (1)、cytochalasin F (2)、cytochalasin B2 (3)were obtained and elucidated from the fungus.