99 / 2023-07-17 17:48:44

Hydrothermal pretreatment enhanced enzymatic hydrolysis of waste tissue paper for bioethanol production

waste tissue paper,hydrothermal pretreatment,lignocellulosic biomass,enzyme hydrolysis,Bioethanol;

Pretreatment plays a crucial role in the resource utilization of lignocellulosic biomass. In this study, Tissue paper (TP) was selected as a representative waste paper, and a pretreatment method using high-pressure hydrothermal and various solvents (water, 1%w/v NaOH or DES) was evaluated. The effects of pretreatment solvent, pretreatment temperature and pretreatment time on the physicochemical properties and enzymatic hydrolysis of TP were systematically studied. Due to the effective delignification under high pressure conditions, the sensitivity of the raw material to further degradation can be significantly improved. The presence of 68.42±3.42% carbohydrates (all cellulose) makes paper towels a potential renewable biomass for bioethanol production. The paper towel was found to contain cellulose (59.60±2.98%), hemicellulose (8.82±0.44%), lignin (29.93±1.50%) and ash (0.37±0.019%). The optimal condition of high-pressure hydrothermal pretreatment of waste paper towels is to react with pure water as a solvent in a high-pressure reactor for 50 minutes at 160℃, maintain the ratio of biomass to water at 1:25 (w/v), and recover glucose and xylose from the hydrolysate of waste paper. Simultaneous saccharification fermentation and distributed saccharification fermentation of waste paper hydrolysate were used in the mixture of saccharomyces suhatasensis and saccharomyces cerevisiae.