314 / 2024-09-14 06:39:17

Dynamics of microbial metabolism on marine snow organic matter

Marine snow,Marine snow degrading microbes,Free-living and marine snow-attached microbes,Metagenomics,Metaproteomics

Background

Marine snow represents an organic matter-rich habitat and provides substrates for diverse microbial populations in the marine ecosystem. However, the functional diversity and metabolic interactions within the microbial community inhabiting marine snow remain largely unclear. Here, we used a multi omics approach to explore the microbial response to marine snow in a simulated marine snow system.

Results

Our results demonstrated a dramatic shift in both taxonomic and functional profiles of the microbial community after marine snow formation. The changes in microbial metabolic processes were more dynamic in the metaproteome than in the metagenome in response to marine snow. Fast-growing taxa within the Gammaproteobacteria were the most dominant group at both the metagenomic and metaproteomic level. These Gammaproteobacteria possessed a variety of carbohydrate-active enzymes (CAZymes) and transporters facilitating substrate cleavage and uptake, respectively. Analysis of metagenome-assembled genomes (MAGs) revealed that the response to marine snow amendment was primarily mediated by Alteromonas, Vibrio, and Thalassotalea. Among these, Alteromonas exclusively expressing CAZymes were abundant in both, the free-living (FL) and marine snow-attached (MA) microbial communities. This suggested a key role of Alteromonas as “pioneers” in initiating the degradation of marine snow. The CAZymes produced by these Alteromonas MAGs are capable of breaking down marine snow into smaller poly- and oligomers, providing available substrates for other microorganisms within the system. Subsequently, Vibrio and Thalassotalea MAGs exhibited distinct responses to these hydrolysis products of marine snow, resulting in a distinct niche separation. Although chemotaxis proteins were found to be enriched in the proteome of all three MAGs, differences in transporter proteins were identified as the primary factor contributing to the niche separation between these two groups. Vibrio in the FL fraction predominantly utilized ATP-binding cassette transporters (ABCTs), while Thalassotalea MAGs in the MA fraction primarily employed TonB-dependent outer membrane transporters (TBDTs).

Conclusions

Our findings shed light on the essential metabolic interactions within marine snow degrading microbial consortia, which employ complementary physiological mechanisms and survival strategies to effectively scavenge marine snow. This work deepens our understanding of the fate of marine snow and the role of microbes in carbon sequestration in the ocean.