摘要详情
984 / 2024-09-19 23:48:27
Hexabromocyclododecane-induced reproductive toxicity in Brachionus plicatilis: Impacts and assessment
HBCD,Marine rotifer,Reactive oxygen species,Oxidative stress,Apoptosis,Risk assessment
摘要待审
Hexabromocyclododecane (HBCD), an emerging pollutant, has aroused worldwide concern because of its wide application and potentially negative impacts on marine ecosystems, but an information gap still exists regarding marine low-trophic organisms. Brachionus plicatilis, the model marine zooplankton, was used in the present study, and its reproductive responses were used as the endpoint to indicate HBCD-induced toxicity. HBCD was suggested to be extremely highly toxic compounds regarding the 96 h-LC50 of 0.58 mg L-1. The sublethal exposure of HBCD injured the reproduction of B. plicatilis: The total number of offspring per female and the key population index calculated from the life table, including the intrinsic rate of population increase (rm) and net reproductive rate (R0), were significantly influenced in a concentration-dependent manner. The reproductive process was also altered, as indicated by the first spawning time, first hatching time and oocyst development time. At the same time, individual survival and growth (body length) were also negatively affected by HBCD. Reactive oxygen species (ROS) were suggested to be responsible for reproductive toxicity mainly because the total ROS contents as well as the main components of •OH and H2O2 greatly increased and resulted in the oxidative imbalance that presented as malondialdehyde (MDA) elevation. Simultaneous activation of the glutathione antioxidant system was accompanied by the apoptosis marker enzymes Caspase-3 and 9, as well as the correlation between ROS content, physiological alteration and cell apoptosis, providing further evidence for this. The integrated biomarker response (IBR) and adverse outcome pathway (AOP) showed that HBCD had a significant toxic effect on B. plicatilis near the concentration range of 96 h-LC50. The establishment of this concentration range will provide a reliable reference for future environmental concentration warning of HBCD in marine.
重要日期
-
会议日期
01月14日
2025
至01月17日
2025
-
09月27日 2024
初稿截稿日期
-
12月14日 2024
注册截止日期
主办单位
State Key Laboratory of Marine Environmental Science, Xiamen University
联系方式
- JKejing GU
- xm******@xmu.edu.cn
- +86*********
