Bioimaging has continued to evolve across a wide spectrum of applications from diagnostics to personalized therapy to mechanistic understanding of biological processes. The field continues to be challenged with ever increasing demand on more robust methods and integration with clinical and molecular data. The 2013 IEEE International Symposium on Biomedical Imaging will be held April 7-11, at the Westin Hotel in San Francisco, CA. ISBI is a joint initiative of the IEEE Signal Processing Society (SPS) and IEEE Engineering in Medicine and Biology Society (EMBS). ISBI has a strong tradition of fostering integration between different imaging communities and of contributing to an integrative imaging approach accessing all scales of observation. We are soliciting all aspects of biological and medical imaging, from mathematical image reconstruction for medical applications to integration of image-based modeling with genomic data for hypothesis generation and network inference. The meeting will feature preconference tutorials, followed by a strong scientific program of plenary talks and special sessions as well as oral and poster presentation of peer-reviewed contributed papers.