The 2016 Gordon Research Conference on Notch Signaling in Development, Regeneration and Disease will provide a stimulating forum to discuss and disseminate new research, concepts and therapeutic opportunities at the forefront of Notch-related biology. Although the first "Notch phenotype" was described over a century ago, the central importance of the Notch pathway in many cell fate decisions, and in a wide range of human diseases is now established: mutations of core Notch pathway components underlie developmental syndromes, adult onset diseases, and cause or contribute to cancer. This meeting will stimulate cross-disciplinary discussions and collaborations among participating scientists in this critically important area of developmental biology and signaling, and nurture a developing sense of community among researchers in the field. The meeting will bring together scientists addressing fundamental mechanistic, developmental, clinical and therapeutic questions using diverse approaches. The invited participants include a blend of established and early career scientists with exciting new findings. Time is also set aside for short talks by poster presenters. The collegial and confidential atmosphere of this conference, with programmed discussion sessions as well as opportunities for informal gatherings in the afternoons and evenings, provides an ideal environment for free scientific exchange. The inclusion of a Gordon Research Seminar (GRS) exclusively for postdocs and graduate students prior to the main conference will also draw new scientists into the field and encourage participation from trainees at all levels.